23 0 obj<> endobj (1951, J. Biol. 0000006310 00000 n Specifications . Keep eye wide open while rinsing. The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. Exoproteins from the indicated strains were quantified by the bicinchoninic acid assay, loaded in equal amounts, separated on 12% SDS-polyacrylamide gels, Techniques Used: Expressing, Acid Assay 6) Product Images from "CRISPR editing of mutant IDH1 R132H induces a CpG methylation-low state in patient-derived glioma models of G-CIMP" .. The Bicinchoninic Acid Assay (BCA), also referred to as the Smiths' Assay, is a biochemical assay designed by Paul Smith in 1985. Search. 0000001086 00000 n C of A & Other Certificates. The principle of the BCA assay relies on the formation of a Cu2+ protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu+. 0000015256 00000 n 0000004532 00000 n xref 0000014295 00000 n 0000006069 00000 n Download SDS (MSDS) Region ... 2,2prime-Bicinchoninic Acid (CAS 1245-13-2) Product Citations See how others have used 2,2prime-Bicinchoninic Acid (CAS 1245-13-2). <]>> View product specific information, MSDS, References and Buying FAQ. Eye Contact Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Click on the entry to view the PubMed entry . Please Contact Us for more information. The BCA Protein Assay is a detergent compatible formulation based on Bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. The Bicinchoninic Acid (BCA) Protein Assay primarily relies on two reactions. Firstly, the peptide bonds in the protein sample reduce Cu 2+ ions, in a temperature dependent reaction, from the copper Bicinchoninic acid / baɪsɪnkɔːnɪnɪk / is a weak acid composed of two carboxylated quinoline rings. Bicinchoninic acid is most commonly employed by biochemists in the bicinchoninic acid (BCA) assay, which is used to determine the total level of protein in a solution.In this assay, two molecules of bicinchoninic acid chelate a single Cu + ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm. ZERO BIAS - scores, article reviews, protocol conditions and more Specifications . The BCA Protein Assay is a detergent compatible formulation based on Bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. ��6�f�A��vD �J����Q��Ѳ~��[�o ��\�i]����OX�r�"��Ƕ�h9�"i#�*�3;H��}pF�� t�����ۖ����� Description: Bicinchoninic acid disodium salt, Cas NO: 979-88-4, Molecular formula: C20H10N2O4Na2, ... SDS. 0000003471 00000 n safety data sheet cat# k154-2500, -5000, bca protein assay kit ii sds date: jun 4, 2015 section 1: product and company identification . Exoproteins from the indicated strains were quantified by the bicinchoninic acid assay, loaded in equal amounts, separated on 12% SDS-polyacrylamide gels, Techniques Used: Expressing, Acid Assay 6) Product Images from "CRISPR editing of mutant IDH1 R132H induces a CpG methylation-low state in patient-derived glioma models of G-CIMP" of reagents and the overall simplicity of the … In particular, it is insensitive to detergents such as Triton X-100 and SDS (1 %). 0000014025 00000 n The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. 0000005828 00000 n Search. The BCA Protein Assay combines the well-known reduction of Cu 2+ to Cu 1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu 1+) by bicinchoninic acid (BCA). Download SDS (MSDS) Region ... Bicinchoninic acid disodium salt (CAS 979-88-4) Product Citations See how others have used Bicinchoninic acid disodium salt (CAS 979-88-4). SDS-PAGE and molecular cloning procedures were carried out by standard * This work was supported by National Institutes of Health Grants R29 DK46212 and RO1 AR44838 and grants from the Barnes-Jewish Hospital Foundation. A bicinchoninic acid protein assay kit and glycerol were supplied by Fisher Scientific (UK). Bicinchoninic acid is most commonly employed by biochemists in the bicinchoninic acid (BCA) assay, which is used to determine the total level of protein in a solution. Search results for Bicinchoninic Acid (BCA) at Sigma-Aldrich bicinchoninic acid (see reference 1 on page 4). PRECISIO ® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution; Sigma-Aldrich pricing. The requested SDS is not available. 0000009016 00000 n 0000011909 00000 n 0000010084 00000 n First-aid measures General Advice Immediate medical attention is required. The requested SDS is not available. 0000000016 00000 n [ �@k�� ��Ҁd*HÞ@Z�%�*�x�>�q`avH8���. Search results for Bicinchoninic Acid (BCA) at Sigma-Aldrich 0000005596 00000 n Techniques: Double Staining, TUNEL Assay, Staining, Expressing Bicinchoninic acid solution is used in the bicinchoninic acid (BCA) assay, which is equivalent to the Lowry procedure. 1 Product Result | Match Criteria: Product Name Synonym ... ≥95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture; Sigma-Aldrich Bicinchoninic acid disodium salt hydrate. C of A & Other Certificates. The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cuprous ion. Specification Name Specification Value; Package Contents: BCA Solution, 2 x 250 ml, Copper Solution, 10 ml, Bovine Serum Albumin Standard, 2 mg/ml, 2 x 5 ml 193, 265-275) have been extens … The Bicinchoninic acid method is faster and easier than Lowry, with much greater tolerance to interference from non-ionic detergents and buffer salts. Show this safety data sheet to the doctor in attendance. The requested SDS is not available. View product specific information, MSDS, References and Buying FAQ. Type. Trichloroacetic acid Revision Date 18-Jan-2018 Y1 - Indicates an exempt polymer that has a number-average molecular weight of 1,000 or greater. The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cuprous ion. Trichloroacetic acid 76-03-9 >95 4. Substances used for the detection, identification, analysis, etc. Type Lot Number Please enter Lot Number Incorrect Lot Number is entered. Article Snippet: The One step TUNEL apoptosis assay kit, DAPI, RIPA lysis buffer, an SDS-PAGE kit and bicinchoninic acid (BCA) protein assay kit were purchased from Beyotime Institute of Biotechnology. • Bicinchoninic acid disodium salt hydrate has been used to monitor the levels of reducing sugars, thereby helping in the detection of chitinases. Application. This water-soluble complex exhibits a strong absorbance at 562 nm that is nearly linear with increasing protein concentrations over a broad working range (20–2000 µg/mL). 0 Type Lot Number Please enter Lot Number Incorrect Lot Number is entered.

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